Abstract
The integration of nanomaterial with biology is finding wide applicability in various areas of medical sciences. Recently, silver nanoparticles have gained attention because of their antimicrobial activity which offers the possibility of their use for medical purposes. Metallic nanoparticles can be obtained by physical, chemical or biological methods. But silver nanoparticles synthesized by chemical reduction methods are found to be toxic, flammable and not at all eco-friendly. Therefore in the present study, an attempt was made to formulate a cost effective and environment friendly technique for green synthesis of silver nanoparticles. The silver nanoparticles were synthesized using extract of Phyllanthus amarus with AgNo3 solution . Their antibacterial activity was studied using agarwell diffusion method on isolates viz., Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, Enterobacter obtained from patients suffering from urinary tract infection. The antibacterial efficacy of silver nanoparticles of Phyllanthus amarus was found to be superior to that of hot or cold extracts of Phyllanthus amarus silver nanoparticles were characterized by UV-Vis spectroscopy and scanning electron microscopy (for particles size analysis). This work demonstrates the possible use of biologically synthesized silver nanoparticles to treat urinary tract infections.