Abstract
Protease have a characteristic of biotechnological interest and have become industrial enzyme. The present study was undertaken to isolate and characterize the protease producing Bascillus subtilis bacteria from gut of fresh water fish Puntius ticto. Gelatin was used as the substrate in nutrient agar medium for screening and showed the maximum zone of activity (21mm) after overnight incubation and addition of the indicator in .The bacterial isolate were identified on the basis of characterized by morphological and biochemical studies. The extracellular protease production was enhanced by optimizing the culture condition. The effect of different production parameter such as temperature of 30̊ C, pH -8.0, incubation time 36 hrs, 1% starch and 1% yeast extract were optimized for maximum yield of protease enzyme.

201604025 ISOLATION AND SCREENING OF PROTEOLYTIC BACTERIA FROM FRESH WATER