IN VITRO ANTIOXIDANT ACTIVITY, TOTAL PHENOL AND TOTAL FLAVONOID CONTENT OF Orthosiphon aristatus (Blume). Miq.

Abstract
The aim of this was to examine the antioxidant activity, total phenol and total flavonoid content of the leaf of Orthosiphon aristatus collected from three distant regions of Tamil Nadu namely Azhizar, Trichy, Chengalpattu. These were examined using extracts of aqueous, ethanol, acetone, chloroform and petroleum ether. The leaf extracts were evaluated for antioxidant activities by DPPH (1,1 – diphenyl -2- picryl-hydrazyl) radical scavenging assay. Among three accessions with different solvents used, maximum antioxidant activity was found in ethanolic leaf extract 85.2% from cuddalor followed by others. Total phenol and flavonoid content was quantitatively estimated, the ethanolic leaf extract of Orthosiphon aristatus was found maximum in Azhiyar accessions (17.13 mg Gallic Acid Equivalents (GAE)/g and 9.30 mg Quercetin Equivalents (QE) /g). The powerful antioxidant activity is attributed to the greater amount of total phenol and flavonoid compound in the ethanolic leaf extract ofOrthosiphon aristatus

2017050106-IN VITRO ANTIOXIDANT ACTIVITY, TOTAL PHENOL AND TOTAL FLAVONOID CONTENT OF Orthosiphon aristatus (Blume). Miq pdf. ch

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