The present investiction deals with the utilization of different concentration (control, 5, 10, 25, 50, 75 & 100 %) of sugar mill effluent on growth and biochemical contents of blackgram (Vigna mungo, var.KM-2). The physico chemical properties of the effluent was brown in colour with acidic in nature. BOD and COD values in high and rich in suspended and dissolved solids. It also considerable quantity of nutrients also present. The results shows that the lower concentration 10 per cent were increased the growth and biochemical contents. The above mentioned parameters were decreased with the increase of effluent concentrations. However, the lower concentration promote the crops plants.