With increasing industrial development the disposal of industrial effluent has become the more ecological challenge.The present investigation was carried out to screen the effect of dairy effluent on the germination and pigment contents of Phaseolus trilobus(Ait). The findings revealed a remarkable effect in germination with treated dairy effluent on morphological and biochemical changes of narepayeru(Phaseolus trilobus) var.Co-2 . The physico-chemical analysis of the diary effluent were analysed . It showed high alkalinity, BOD, COD values and more total dissolved salts. Effect of various treatments like 10,25,50,75 and 100 per cent of the effluent on seed germination and biochemical changes of Phaseolus trilobus seedlings. The results indicated that the lower concentrations, there was a significant increase in the seed germination and other growth parameters, but it decreased with increased above 25 per cent of effluent treatment. At lower concentrations it stimulated the germination, growth of root and shoot lengths and biochemical changes. Therefore it can be used as a liquid fertilizer.