Abstract
An efficient system for propagation of Dendrobium densiflorum Wall. a medicinally important orchid, through in vitro symbiotic seed germination has been established. Mature seeds from 8months old green pods of Dendrobium densiflorum were inoculated on different culture media viz., Murashige and Skoog (MS), Nitsch and Nitsch (NN), Gamborg et al. (B5) and Knudson C (KC) without phytohorrmone supplementation. MS medium proved to be the best as about 94% of the seeds gave complete plantlets with well-developed roots and shoots within 90 days. About 7 months-old plantlets hardened in a compost mixture comprising brick, charcoal, decaying litter in a ratio of 1:1:1 and a layer of moss on top showed survival percentage of 71.

201604027 CONSERVATIONAND MASS PRODUCTION OF DENDROBIUM DENSIFLORUM WALL–IN VITRO pdf