In vitro clonal propagation of vulnerable medicinal plant, Mimosa pudica L. Ramesh.S1, Chandran.C1,Venkatesan.G2.

Mimosa pudica L. is a medicinal herb. The in vitro regeneration protocol of this plant through shoot tip and nodal, Root tip explants. The explants were culured on MS medium supplemented of different concentrations Benzyl amino acid purine (BAP), Kinetin (KN), 2,4-Dichlorophenoxy acetic acid (2,4-D), IAA, NAA, IBA and GA3 were (0.5 to 2.5 mg/l) used. Elongation of regenerated shoots tip, nodel, shoot tip using different growth regulators. Induction of roots from the regenerated shoots. Hardened regenerant were acclimatized to the soil.

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Diversity of soil mycoflora of JatrophacurcasL. Plantation of TamilNadu, Southern India.

A total of number of 57 taxa were isolated by Warcup and Dilution plating method from the soil of Jatrophacurcas  (Biodiesel) plantation at Mannargudi (Thiruvarur Dt.) located in south east cost of India. 57 taxa beloning to 37 genera were isolated and identified from the plantation soil. Several species belongs to various groups viz. one species belonging to Ascomycete, one species from Coleomycete, 47 species from Hyphomycetes remaining 3 species from Zycomycetes and some yeast forms, sterile colonies were recorded. Hyphomyctes groups of  fungi were showed  higher diversity.The Physico-chemical analysis of the soils revealed the following features: Soil texture was Sandy Loam, Calcium Carbonate not contain, pH 6.8, Salt EC(electrical conductivity) 0.09 dSm-1. Macro nutrients (Kg/acre) of the soil were Organic carban 0.06, Nitrogen(N) low, Phosphorus(P) 2.6, potassium(K) 105. Micro nutrients were available Ferrous (F) 17.12 ppm, Manganese(Mn) 8.66 ppm, Zinc(Zn) 0.91 ppm, Copper(Cu) 3.84 ppm and Boron(B) low ppm.

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EFFICACY OF SUGARMILL EFFLENT ON GROWTH AND BIOCHEMICAL CONTENTS OF BLACKGRAM [Vigna mungo(L). Hepper]

The present investiction deals with the utilization of different concentration (control, 5, 10, 25, 50, 75 & 100 %) of sugar mill effluent on  growth and biochemical contents  of blackgram (Vigna mungo, var.KM-2).  The physico chemical properties of the effluent was brown in colour with acidic in nature.  BOD and COD values in high and rich in suspended and dissolved solids.  It also considerable quantity of nutrients also present.  The results shows that the lower concentration 10 per cent were increased the growth and biochemical contents.  The above mentioned parameters were decreased with the increase of effluent concentrations. However, the lower concentration  promote the crops plants.

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Effect of Seaweed Liquid Fertilizer of CaulerpaScalpelliformis on Growth and Enzymatic Activity of AbelmoschusEsculentus L.

The effect of seaweed liquid fertilizer (SLF) of Caulerpascalpelliformis was tested at different concentrations (5, 10, 25, 50, 75, and 100%) on growth and biochemical, and enzymatic activity of Abelmoschusesculentus. The seaweed extract was found effective in increasing the biomass growth of shoot, root length fresh, dry weight pigmentation of chlorophyll-a,b and carotenoids. The biochemical tested like    protein, amino acids. The enzymatic activity like, α, ß amylase, catalase, peroxidase, polyphenol oxidase. The finding of the present study and biochemical, enzymatic activity than that of higher concentration.

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EFFICACY OF SAGO FACTORY EFFLUENT ON SOME VARIETIESOF BLACKGRAM(Vigna mungoL.Hepper)

The germination percentage, seedling growth and dry weight of six varieties of blackgram were high at 10 percent of sago factory effluent for all the varieties when compared to control.  Among the six varieties studied, Vamban-2 showed better growth, the order of tolerance being Vamban-2>Vamban-1>ADT-4>ADT-5>KM-1>ADT-3.

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Successional pattern of mycoflora associated with decomposing leaf litter of Jatrophacurcas L. (Biodiesel plant).

The biodiversity of mycoflora associated with decomposing leaf litter of Jatrophacurcas(Biodiesel plant) and the pattern of fungal colonizationwas studied. Fungi colonizing decomposing litter were induced to sporulate by a moist chamber incubation technique. A total of 79 fungal species belonging to 59 genera were isolates and 4 main groups of taxa were identified. The primary colonizers were mainly saprophytic fungi which survived well on undecomposed litter. The litter was dominated by numerous Hyphomycete and the fungal species of this substrate changed with progressive decomposition. After few days some Ascomycetes, Coeleomycetes and Zycomycetes fungi colonies were dominated. The litter fungi showed a higher diversity in both seasons. There was little overlap between the fungi assemblages of the air and soil group of fungi. The litter fungi showed that nature of the host tissue as well as the environment determines the fungi decomposition of a host.

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MEDICINAL FLORA OF CAUVERY DELTA REGION OF KUTTALAM TALUK, NAGAPATTINAM DISTRICT OF TAMILNADU

Plants have been used as medicine throughout the world, more than five hundred and still to occupy an important place in traditional as well as modern system of medicine. Thousand plants have been used in Indian system of medicines.Most of drug obtained from wild plants.Kuttalam taluk,Kadamangudi is situated in the bank of river Nandalaru a branch of  river CaveryNagapattinam district,Tamilnadu.The river belt have rich in various medicinal plants.Frequent field survey were undertaken during October 2009 to October 2010.About 242 species have been identified from the study area of these all plants are medicinal value in various ailments to cure for remedial treatments. Among the survey, the family Solanaceae having ten rare and indigenous plants

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Fungi associated with the leaves of some Hydrophyte plants

Endophytic and phylloplane fungi were isolated from 10 hydrophyte plants of belonging to families. These plants were collected from different location in Tamil Nadu of the 3000 leaf bits was analyzed only few of endophytic fungal species were fount to colonized. Among these four belonging to Ascomycetes, nine belonging to Coelomycetes and twenty five Hyphomycetes beside to two sterile forms. A comparison of the phylloplane flora with endophytic fungi of the host studied shows that some of invading the leaves tissue those forming endophytic association.

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Effect of Seaweed Liquid Fertilizer of Sargassummyryocystem ofAbelmoschusesculentus(L.)

The seaweed liquid fertilizers (SLF) was obtained from Gulf of Mannar (Monnali Island) Tamil nadu and tested on the vegetables crops Abelmoschusesculentus (Bhendi). The seeds were obtained from US Agri seeds F1 HYPRID in India Pvt Ltd. Seeds of Abelmoschusesculentus were treated in diffeent concentration such as 5, 10, 25, 50, 75 and 100% of Sargassummyryocystem SLF. The plants raised from seeds treated with 10% Sargassummyryocystem SLF showed and increase in fresh weight, dry weight, root and shoot length and total chlorophyll, chlorophyll-a, chlorophyll-b, carotenoids, protein and amino acids.

Effect of Seaweed Liquid Fertilizer of Sargassummyryocystem ofAbelmoschusesculentus(L.)

Effect of diary effluent on seed germination and pigment contents of Phaseolus trilobus(Ait)

With increasing industrial development the disposal of industrial effluent has become the more ecological challenge.The present investigation was carried out to screen the effect of dairy effluent on the germination and pigment contents of Phaseolus trilobus(Ait).  The findings revealed a remarkable effect in germination with treated dairy effluent on morphological and biochemical changes of narepayeru(Phaseolus trilobus) var.Co-2 .  The physico-chemical analysis of the diary effluent were analysed .  It showed high alkalinity, BOD, COD values and more total dissolved salts.  Effect of various treatments like 10,25,50,75 and 100 per cent  of  the effluent on seed germination and biochemical changes of Phaseolus trilobus seedlings. The results indicated that the lower concentrations, there was a significant increase in the seed germination and other growth parameters, but it decreased with increased above 25 per cent  of effluent treatment.  At lower concentrations it stimulated the germination, growth of root and shoot lengths and biochemical changes. Therefore it can be used as a liquid fertilizer.

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