TOXIC EFFECT OF LEAD NITRATE [Pb(NO3)2]ON THE BLACK GRAM SEEDLINGS (Vigna mungo (L.)Hepper)

Abstract
Heavy metals are integral components of ecosystem. The distinctivenesscharacteristic of heavy metal is poisoning and resulting in the inactivation of enzyme systems. All heavy metals are potentially toxic at elevated concentrations. In the present investigation, effect of Lead nitrate[Pb (NO3)2], on the morphological parameters of black gram seedlings (Vigna mungo (L.)Hepper),the germination percentage and morphological parameters such as, root length, shoot length, number of leaf, total leaf area, root nodules,fresh and dry biomass were analyzed on the 15th days of seedlings. Among the all parameters were reduced in increasing the Pb(NO3)2 concentration when compared to control plants.

A14.TOXIC EFFECT OF LEAD NITRATE [Pb(NO3)2]ON THE BLACK GRAM SEEDLINGS

Preliminary Phytochemical Screening and Antimicrobial activity of Pterolobium hexapetalum (Leguminosae)

                                                            Abstract

An ethnomedicinal plant, Pterolobium hexapetalum (Roth) Santapau & Wag (Leguminosae) was analyzed for antimicrobial activity. Bioassay of antimicrobial activity of hexane, chloroform and ethanol leaf extract showed significant activity against the human pathogens such as Proteus vulgaris, Pseudomonas aeruginosa causing urinary tract infections and septicaemia, Salmonella typhi causing typhoid fever, Vibrio species causing diarrheal infections and the fungus Candida albicans. The antimicrobial activity expressed the concentration-dependent against all the tested microorganisms.  Ethanol extracts exhibited the highest antimicrobial activity. The present findings provide an authentic scientific evidence for the traditional usages by various tribes in India. The bioactive compounds responsible for these antimicrobial activities against the tested microorganisms should be isolated and identified to develop a new lead of therapeutic interest.

A6Preliminary Phytochemical Screening and Antimicrobial activity pdf

Pollen analysis of honey samples from Western Ghats, Tamil Nadu, India

Abstract

    The present research work is about the melissopalynological studies of from honey samples of Apis florae collected from different places of Western Ghats. All the samples of multifloral type around 32 pollen morphotypes are observed from there four honey samples. Pollen of Acacia is the one represented in all the samples. Among the four honey samples H1 shows the maximum number (22) of pollen morphotypes to conclude this study shows the forage behavior of Apis florae.

A5Pollen analysis of honey samples from Western Ghats pdf

 

Role of calcium carbonate on growth, membrane stability and nutrient uptake of Pennisetum americanum (L. ) Leeke grown under NaCl stress.

                                                     Abstract

A field experiment was carried out the pearl millet (Pennisetum americanum L., Leeke) in a mixture of garden soil, manure, and sand (1:1:1) to investigate the effects of supplementary calcium carbonate on plants grown at high NaCl concentration (50 mM). The treatments were: (i) control, (ii)NaCl treatment alone (50 mM); (iii) combined salt (50mM NaCl+10mM CaCo3). The plants grown under NaCl stress produced low fresh and dry weight and relative water content that those grown in control plants. Supplemental calcium carbonate added to NaCl treated plants significantly improved the growth and physiolocal differences affected by stress (e.g. plant growth, and membrane permeability) and also increase the K+ , Ca2+ , and N content in pearl millet. The effects of supplemental CaCO3 in maintaining membrane permeability, increase the content of  Ca2+ , N and K+  and reduce the content of Na+ in pearl millet crops production caused by high NaCl salinity.

A3 Role of calcium carbonate on growth, membrane stability pdf

 

Effectiveness and efficiency of Chemical and Physical Mutagens in Sorghum (Sorghum bicolor (L.)Moench) in M2 generation

Abstract

The effectiveness and efficiency of Chlorophyll mutants were observed in treated material of Sorghum with gamma rays and EMS. The study was undertaken in sorghum (Sorghum bicolor (L.) Moench) variety CSV-23 to access the efficiency and effectiveness of Physical and chemical mutagens. EMS treatment were found highly effective than the gamma rays. Mutagened effectiveness and efficiency decreased with increased in all mutagenic treatments. In general the mutation frequency was high in M1 plants than M2.

A 1Effectiveness and efficiency of Chemical and Physical Mutagens pdf

 

Effect of lead on total chlorophylls and proline content of Dolichos biflorus (L.)

Abstract
The effects of Lead acetate [Pb(CH3COO)2] content of chlorophyll and proline were investigated in 15-to 75 days Fabaceae member plant of Dolichos biflorus (L.) in lead acetate treatment of Control to 10,25,50,75,100 and 200 mg/kg soil with various concentration of [Pb(CH3COO)2]. The control to pb treated 15 to 75 -days plants were taken and measured for total chlorophyll content of “aˮ and“ b”. The proline content was measured by using spectrophotometer under 520 nm. The total chlorophyll content was decreased with increased concentration of pb treatment, whereas proline content was measured on 15to 75 days plant of after lead acetate treatment. The bio-chemical content of total chlorophyll and proline was significantly affected with treatment of [Pb(CH3COO

A15.Effect of lead on total chlorophylls and proline content pdf.

Effect of cadmium contaminated soil on germination and seedling growth of Jatropha curcas. (Linn.)

Abstracts
A pot culture experiment, conducted Jatropha curcas. (L.) to germination and seedling growth the metal cadmium was added in the form of cadmium chloride. Different concentrations of cadmium (C, 10, 20, 40, 60,100 and 200 mg kg-1 soil) were prepared and used for germination studies. Plants were grown up to 30 days. The inner surface of pots was lined with a polythene paper. Each pot contained 5kg of air dried soil. The 10 seeds were sown in each pot. All pots were irrigated daily. In the studies from the results obtained that the increase in cadmium concentration affected all the growth parameters such as germination percentage, length of root and shoot, number of leaves, fresh weight of root, shoot and leaf, vigour index and tolerance index.

Studies on the effect of mutagens on seed germination, survival and pollen sterility in M1- generation of Sesame (Sesamum indicum L.)var.TMV-3.

Abstract
This study was aimed to compare the effect of physical and chemical mutagens, namely gamma rays and EMS on seed germination, survival of seedlings and pollen sterility of sesame genotype TMV3. The seeds of sesame variety TMV3 were subjected to different doses of gamma rays such as 10, 20, 30, 40, 50, 60, 70, 80, 90, 100KR and various concentrations of EMS like 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6, 1.8 and 2.0mM. Seed germination test was carried out in laboratory to find out the LD50 values of Gamma rays and EMS and they were 50KR for gamma rays and 1.0mM for EMS. Based on the LD50 values, the following doses and concentrations were chosen to treat the seeds and conduct the field experiments. For Gamma rays, they were 30, 40, 50, 60 and 70KR and for EMS they were 0.6, 0.8, 1.0, 1.2 and 1.4mM and for Combined treatments they were 30KR+0.6mM, 40KR+0.8mM, 50KR+1.0mM, 60KR+1.2mM and 70KR+1.4mM. The effect of mutagens was measured for quantitative characters like seed germination, seedlings survival, seedlings height and pollen sterility and calculations were made. The results indicated that the seed germination, seedling survival and seedling height were decreased with increasing doses or concentrations of mutagens when compared to control. But in case of pollen sterility, it was increased with increasing concentrations of mutagens when compared to control. In combined treatments, plants showed high percentage of pollen sterility when compared to individual treatments and some plants showed complete sterile at 70KR + 1.4mM treatment.

IN VITRO PRODUCTION OF INDOLE ACIDIC ACID AND BIOACTIVITY OF FUNGAL ENDOPHYTES FROM MEDICINAL PLANTS.

Abstract
Endophytic microorganisms are to be found in almost every plant on earth. These organisms reside in the living tissues of the host plant and do so in a variety of relationships ranging from symbiotic to pathogenic. The aim of the present work was to investigate the fungal endophytes from the medicinal plant Argemone Mexicana.L and Aristolochia baetica.L. The isolate fungal endophytes and might be producing reasonably and pharmaceutically important bioactive (IAA, Enzymes) compounds from these plants. The determine of the antifungal activities of methanol, ethanol chloroform and aqueous extracts of Aristolochia baetica. Leaf against fungi by agar disc diffusion method. The antifungal assay was done against endophytic fungi (Aspergillus niger, Aspergillus flavus, Penicillium sp.). For all the cases it showed significant result.

Detection of Antimicrobial activity from Mimosa pudica L.

Abstract
This paper deals with the systematic studies on antifungal and antibacterial activity from Mimosa pudica L. We are analyze the shade dried and powdered plant materials were extracted with methanol, chloroform, ethanol using a soxhlet apparatus and water extract was centrifuged at 5000 rpm were using supernatant. Gram negative bacteria strains of Escherichia coli, Klebsiella pneumoniae and Gram positive bacteria of Staphylococcus aureus, Streptococcus pyogensis, and Bacillus subtilis and the fungal strains of Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, Aspergillus awamori and Penicillium janthinellum were studied antimicrobial assay by using disc diffusion method. The different concentrations of the extract to understand the most effect microbial activity of microorganism