The biodiversity of mycoflora associated with decomposing leaf litter of Jatrophacurcas(Biodiesel plant) and the pattern of fungal colonizationwas studied. Fungi colonizing decomposing litter were induced to sporulate by a moist chamber incubation technique. A total of 79 fungal species belonging to 59 genera were isolates and 4 main groups of taxa were identified. The primary colonizers were mainly saprophytic fungi which survived well on undecomposed litter. The litter was dominated by numerous Hyphomycete and the fungal species of this substrate changed with progressive decomposition. After few days some Ascomycetes, Coeleomycetes and Zycomycetes fungi colonies were dominated. The litter fungi showed a higher diversity in both seasons. There was little overlap between the fungi assemblages of the air and soil group of fungi. The litter fungi showed that nature of the host tissue as well as the environment determines the fungi decomposition of a host.