Mimosa pudica L. is a medicinal herb. The in vitro regeneration protocol of this plant through shoot tip and nodal, Root tip explants. The explants were culured on MS medium supplemented of different concentrations Benzyl amino acid purine (BAP), Kinetin (KN), 2,4-Dichlorophenoxy acetic acid (2,4-D), IAA, NAA, IBA and GA3 were (0.5 to 2.5 mg/l) used. Elongation of regenerated shoots tip, nodel, shoot tip using different growth regulators. Induction of roots from the regenerated shoots. Hardened regenerant were acclimatized to the soil.

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